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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in several tumors, which leads to chromosome instability, and therefore may well lead to tumorigenesis. This protein was uncovered to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been described. Two more splice variants have been reported but thorough nucleotide sequence information just isn't still accessible.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged with the antibody.
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This manual outlines more possible optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide no more bind into the epitope around the concentrate on protein. By evaluating the staining through the blocked antibody as opposed to the antibody on your own, one can see which staining is specific.
Target Information and facts Cyclin E click here belongs into the remarkably conserved cyclin relatives, whose associates are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E sorts a complex with and functions as a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S changeover. This protein accumulates on the G1-S stage boundary and is degraded as cells progress by means of S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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